Composite

Part:BBa_K137056:Design

Designed by: Victoria Hsiao   Group: iGEM08_Caltech   (2008-07-31)


folB + folKE + b0015 in psb2k3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The rest of the plasmid includes the constitutive promoter j23100 and the strong RBS b0034 in the inducible copy plasmid psb2k3.


Source

The two genes were cloned from the genome of lactococcus lactis subspe. IL1403

References